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Cellulase production in a new mutant strain of Penicillium decumbens ML-017 by solid state fermentation with rice bran

Paper ID Volume ID Publish Year Pages File Format Full-Text
33489 44980 2011 5 PDF Available
Title
Cellulase production in a new mutant strain of Penicillium decumbens ML-017 by solid state fermentation with rice bran
Abstract

To produce cellulolytic enzyme efficiently, Penicillium decumbens strain L-06 was used to prepare mutants with ethyl methane sulfonate (EMS) and UV-irradiation. A mutant strain ML-017 is shown to have a higher cellulase activity than others. Box–Behnken's design (BBD) and response surface methodology (RSM) were adopted to optimize the conditions of cellulase (filter paper activity, FPA) production in strain ML-017 by solid-state fermentation (SSF) with rice bran as the substrate. And the result shows that the initial pH, moisture content and culture temperature all have significant effect on the production of cellulase. The optimized condition shall be initial pH 5.7, moisture content 72% and culture temperature 30°C. The maximum cellulase (FPA) production was obtained under the optimized condition, which is 5.76 IU g−1, increased by 44.12% to its original strain. It corresponded well with the calculated results (5.15 IU g−1) by model prediction. The result shows that both BBD and RSM are the cellulase optimization methods with good prospects.

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Cellulase production in a new mutant strain of Penicillium decumbens ML-017 by solid state fermentation with rice bran
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 6, October 2011, Pages 733–737
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us