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Significant improvement of Geobacillus thermoleovorans CCR11 thermoalkalophilic lipase production using Response Surface Methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
33493 44980 2011 6 PDF Available
Title
Significant improvement of Geobacillus thermoleovorans CCR11 thermoalkalophilic lipase production using Response Surface Methodology
Abstract

The medium optimization for the production of the Geobacillus thermoleovorans CCR11 thermoalkalophilic lipase was carried out in shake flask cultures using safflower high oleic oil. In the first step of optimization, a two level fractional factorial design allowed the identification of the concentration of nutrient broth and temperature as the main variables significantly affecting lipase production (P < 0.05). In a second step, a D-optimal design was applied to determine the variables optimal values, defined as those yielding maximal lipase production in shaken flasks, thus demonstrating that the optimal concentration of nutrient broth was 3.8 g/l and the optimal culture temperature was 39.5 °C. The model was experimentally validated, yielding a lipase production of 2283.70 ± 118.36 U/mL which represents a 6.7-fold increase in comparison to the non-optimized medium.

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Significant improvement of Geobacillus thermoleovorans CCR11 thermoalkalophilic lipase production using Response Surface Methodology
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 6, October 2011, Pages 761–766
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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