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A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing

Paper ID Volume ID Publish Year Pages File Format Full-Text
33499 44980 2011 8 PDF Available
Title
A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing
Abstract

Although several xylanases have been studied, only few xylanases from marine micro-organisms have been reported. We report here a novel halotolerant xylanase from marine bacterium Bacillus subtilis cho40 isolated from Chorao island of mandovi estuary Goa, India. Extracellular xylanase was produced by using agricultural residue such as wheat bran as carbon source under solid-state fermentation (SSF). The optimal pH and temperature of xylanase were reported to be 6.0 and 60°C, respectively. Xyn40 was highly salt-tolerant, and showed highest activity at 0.5 M NaCl. Xylanase activity was greatly induced (140%) when pre-incubated with 0.5 M NaCl for 4 h. The xylanase gene, xyn40, from marine bacterium B. subtilis cho40 was cloned, and expressed in Escherichia coli. The xylanase gene was 645 bp long and had a 215 amino acid ORF protein with a molecular mass of 22.9 kDa. It had all features of xylanase enzyme and showed homology to xylanases reported from B. subtilis. It differs from the earlier reported xylanase sequences by the presence of more serine residues compared to threonine and also by the presence of polar (hydrophilic) amino acids in higher abundance (61%) than non-polar amino acids (39%). The novel xylanase, reported in this study is a halotolerant enzyme from marine isolate and can play a very important role in bioethanol production from marine seaweeds.

► The xylanase gene, xyn40, from marine bacterium Bacillus subtilis cho40 is of 645 bp and encodes a 215 amino acid protein. ► Xyn40 was highly salt-tolerant, and showed highest activity at 0.5 M NaCl. ► Enzyme activity was greatly induced (140%) when preincubated with 0.5 M NaCl for 4 h. ► Halotolerent xylanase from Bacillus sp. cho 40 can play a very important role in bioethanol production from marine seaweeds.

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A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 6, October 2011, Pages 814–821
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us