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Development and evaluation of data-driven designed tags (DDTs) for controlling protein solubility

Paper ID Volume ID Publish Year Pages File Format Full-Text
33517 44982 2011 7 PDF Available
Title
Development and evaluation of data-driven designed tags (DDTs) for controlling protein solubility
Abstract

Production of proteins is an important issue in protein science and pharmaceutical studies. Numerous protein expression systems using living cells and cell-free methods have been developed to date. In these systems, a promising strategy for improving the success rate of obtaining soluble proteins is the attachment of various tags into target proteins based on empirical rules. This paper presents a method for the production of data-driven designed tags (DDTs) based on highly frequent sequence property patterns in an experimentally assessed protein solubility dataset in a wheat germ cell-free system. We constructed seven proteins combined with 12 kinds of DDTs (six for enhancing solubility and six for insolubility) at the N-terminal region as tags. Then we investigated their behavior using SDS-PAGE. Results show that three and four proteins respectively showed a trend toward solubilization and insolubilization, which indicates the possibility that the theoretically designed sequence can control protein solubility.

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Development and evaluation of data-driven designed tags (DDTs) for controlling protein solubility
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 3, 30 April 2011, Pages 225–231
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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