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Production of membrane proteins without cells or detergents

Paper ID Volume ID Publish Year Pages File Format Full-Text
33520 44982 2011 5 PDF Available
Title
Production of membrane proteins without cells or detergents
Abstract

The production of membrane proteins in cellular systems is besieged by several problems due to their hydrophobic nature which often causes misfolding, protein aggregation and cytotoxicity, resulting in poor yields of stable proteins. Cell-free expression has emerged as one of the most versatile alternatives for circumventing these obstacles by producing membrane proteins directly into designed hydrophobic environments. Efficient optimisation of expression and solubilisation conditions using a variety of detergents, membrane mimetics and lipids has yielded structurally and functionally intact membrane proteins, with yields several fold above the levels possible from cell-based systems. Here we review recently developed techniques available to produce functional membrane proteins, and discuss amphipols, nanodisc and styrene maleic acid lipid particle (SMALP) technologies that can be exploited alongside cell-free expression of membrane proteins.

First Page Preview
Production of membrane proteins without cells or detergents
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 3, 30 April 2011, Pages 250–254
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering