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Reconstitution of the Cpx signaling system from cell-free synthesized proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
33524 44982 2011 5 PDF Available
Title
Reconstitution of the Cpx signaling system from cell-free synthesized proteins
Abstract

Cell-free expression has received growing attention as an effective system to produce integral membrane proteins for biochemical studies. We have applied this technology for the production of the histidine kinase CpxA, an integral membrane sensor that regulates an envelope stress response in Escherichia coli. All phosphotransfer activities of detergent-solubilized CpxA synthesized in vitro have been characterized and compared with those of CpxA solubilized from bacterial membranes. The results demonstrate the simplicity and efficiency of this technology for purifying large quantities of functional membrane proteins.

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Reconstitution of the Cpx signaling system from cell-free synthesized proteins
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 3, 30 April 2011, Pages 277–281
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us