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Cell free expression put on the spot: advances in repeatable protein arraying from DNA (DAPA)

Paper ID Volume ID Publish Year Pages File Format Full-Text
33525 44982 2011 9 PDF Available
Title
Cell free expression put on the spot: advances in repeatable protein arraying from DNA (DAPA)
Abstract

We have previously described the ‘DNA array to protein array’ (DAPA) method for microarraying of proteins expressed by cell-free systems in situ on the array surface. In this technique, a DNA array on one slide acts as the template for generating a protein array on a second slide, mediated by a cell free lysate between the two juxtaposed slides. Here we explore the feature of the repeatability of the technology, in which the same DNA array is reused several times, and use the method to generate a microarray of 116 diverse proteins. The capabilities of DAPA technology in comparison with other protein array methods are discussed.

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Cell free expression put on the spot: advances in repeatable protein arraying from DNA (DAPA)
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 3, 30 April 2011, Pages 282–290
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering