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Isolation and characterization of temperature and alkaline stable bioflocculant from Agrobacterium sp. M-503

Paper ID Volume ID Publish Year Pages File Format Full-Text
33556 44984 2010 6 PDF Available
Title
Isolation and characterization of temperature and alkaline stable bioflocculant from Agrobacterium sp. M-503
Abstract

A bacterium isolated from activated sludge of propylene epoxide wastewater was identified as Agrobacterium sp. M-503. It was confirmed to produce bioflocculant with excellent flocculation activity. The yield of the bioflocculant reached 14.9 g/l in batch cultivation with a carbon source conversion of 74.5%. This bioflocculant was temperature and alkaline stable, retaining almost all flocculation activity after being treated at 121°C for 20 minutes or at pH 12.0. It consisted of neutral sugar, uronic acid, aminosugar and protein in weight ratios of 85.0:9.9:2.1:3.0. The active polysaccharide fraction of the bioflocculant was purified to homogeneity by ethanol precipitation, DEAE ion-exchange and gel chromatography. Analysis of the purified polysaccharide showed that it consisted of glucose residues and had a molecular weight of 8.1 × 104 Da. Its low molecular weight endowed it with excellent solubility and favorable flocculation activity, especially for small particulates.

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Isolation and characterization of temperature and alkaline stable bioflocculant from Agrobacterium sp. M-503
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 27, Issue 6, 31 December 2010, Pages 789–794
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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