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Expression of anti-sclerotinia scFv in transgenic Brassica napus enhances tolerance against stem rot

Paper ID Volume ID Publish Year Pages File Format Full-Text
33560 44984 2010 6 PDF Available
Title
Expression of anti-sclerotinia scFv in transgenic Brassica napus enhances tolerance against stem rot
Abstract

Canola is an important agricultural crop imparting a significant contribution to global oilseed production. As such, optimizing yield and quality is of paramount importance and canola production can be significantly affected by sclerotinia stem rot. The utility of recombinant antibody technology in plant protection has been explored by many researchers and shows promise for the generation of new lines of agriculturally significant crops with greater resistance to diseases. The objective of the current study was to generate recombinant pathogen specific antibody (scFv)-expressing transgenic Brassica napus plants with increased tolerance to the phytopathogenic fungus, Sclerotinia sclerotiorum. Transgenic canola (B. napus) lines expressing S. sclerotiorum-specific scFv antibody showed a significant level of tolerance towards S. sclerotiorum as compared to their non-transformed counterparts. Both incidence and progression of S. sclerotiorum-induced disease symptoms were reduced in plants expressing the recombinant scFv.

First Page Preview
Expression of anti-sclerotinia scFv in transgenic Brassica napus enhances tolerance against stem rot
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 27, Issue 6, 31 December 2010, Pages 816–821
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering