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Purification of fructooligosaccharides in an activated charcoal fixed bed column

Paper ID Volume ID Publish Year Pages File Format Full-Text
33567 44984 2010 8 PDF Available
Title
Purification of fructooligosaccharides in an activated charcoal fixed bed column
Abstract

Fructooligosaccharides (FOS) are mixtures of oligosaccharides containing mono and disaccharides, therefore, the separation of these sugars results in purer products for human consumption and can be added to various food products (drinks, cookies and yogurt). The aim of this work was the purification of fructooligosaccharides from a mixture of sugars, obtained by enzymatic synthesis, containing fructooligosaccharides, glucose, fructose and sucrose using activated charcoal fixed bed column. Temperature and ethanol concentration effects were analyzed using a 22 central composite design. Good separation conditions were obtained through central composite design. The best separation coefficient between fructooligosaccharides and glucose (ESfructoolig/gluc) was 3.99 ± 0.07 and between fructose and fructooligosaccharides (ESfructoolig/fruct) was 2.89 ± 0.36 using ethanol 15% (v/v) as eluent, at 50 °C. The final FOS purification degree and recovery were about 80% and 97.8%, respectively. The activated charcoal fixed bed columns were shown to be a good alternative for sugar separation, especially for rich mixtures of fructooligosaccharides.

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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 27, Issue 6, 31 December 2010, Pages 862–869
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us