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Enhanced production of xylanase by Aspergillus carneus M34 in solid-state fermentation with agricultural waste using statistical approach

Paper ID Volume ID Publish Year Pages File Format Full-Text
33573 44985 2010 8 PDF Available
Title
Enhanced production of xylanase by Aspergillus carneus M34 in solid-state fermentation with agricultural waste using statistical approach
Abstract

The production of a low molecular weight xylanase by Aspergillus carneus M34 was investigated in solid-state fermentation using agricultural waste as the substrate. Experimental designs were practiced for optimisation of the medium composition. When agricultural wastes of coba husk and corn steep liquor were used in the ratio of 4.5:0.5, a 22.5% increase of xylanase activity was observed compared with the medium containing only coba husk. The incubation time for xylanase production can be reduced from 12 days to six days by increasing the inoculum size to 2 × 107 spores mL−1. The optimal media compositions were as follows by using statistical approach: coba husk/corn steep liquor (4.5/0.5); NH4NO3, 32.4 g L−1; CaCl2, 1.34 g L−1; MnSO4·7H2O, 0.0124 g L−1. Xylanase activity of 1721 U g−1 substrate was obtained by A. carneus M34 in a six-day period at 30 °C, which was a 227% increase compared with that obtained before applying the Plackett–Burman and response surface methodology experimental design.

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Enhanced production of xylanase by Aspergillus carneus M34 in solid-state fermentation with agricultural waste using statistical approach
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 27, Issue 1, 28 February 2010, Pages 25–32
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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