fulltext.study @t Gmail

Optimized enzymatic synthesis of caffeic acid phenethyl ester by RSM

Paper ID Volume ID Publish Year Pages File Format Full-Text
33583 44985 2010 5 PDF Available
Title
Optimized enzymatic synthesis of caffeic acid phenethyl ester by RSM
Abstract

In this study, optimization of enzymatic synthesis of caffeic acid phenethyl ester (CAPE), catalyzed by immobilized lipase (Novozym® 435) from Candida antarctica was investigated. Novozym® 435 was used to catalyze caffeic acid and 2-phenylethanol in an isooctane system. Response surface methodology (RSM) and 5-level-4-factor central-composite rotatable design (CCRD) were employed to evaluate the effects of synthesis parameters, such as reaction temperature (30–70°C), reaction time (24–72 hours), substrate molar ratio of caffeic acid to 2-phenylethanol (1:10–1:90) and enzyme amounts (100–500 PLU) on percentage conversion of CAPE by direct esterification. Reaction temperature and time had significant effects on percent conversion. On the basis of ridge max analysis, the optimum conditions for synthesis were: reaction time 59 hours, reaction temperature 69°C, substrate molar ratio 1:72 and enzyme amount 351 PLU. The molar conversion of predicted values and actual experimental values were 91.86 ± 5.35% and 91.65 ± 0.66%, respectively.

First Page Preview
Optimized enzymatic synthesis of caffeic acid phenethyl ester by RSM
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 27, Issue 1, 28 February 2010, Pages 89–93
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us