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Purification of transgenic tobacco-derived recombinant human monoclonal antibody

Paper ID Volume ID Publish Year Pages File Format Full-Text
3364 166 2013 9 PDF Available
Title
Purification of transgenic tobacco-derived recombinant human monoclonal antibody
Abstract

Purification of recombinant monoclonal antibody from transgenic plant extract is technically challenging as it involves the processing of large volume of material, containing low titre of antibody, present along with large quantities of native proteins and other impurities. The conventional approach of capturing antibody from a clarified extract using packed-bed chromatography is therefore not particularly suitable. This study evaluates the suitability of using a combination of ultrafiltration and chromatography for purifying transgenic tobacco-derived human monoclonal antibody. A two-stage cascade ultrafiltration process removed about 97% impurities while ensuring almost complete recovery of antibody, providing 32-fold antibody enrichment in the process. The primary objective of the ultrafiltration step was to reduce the burden on the subsequent chromatographic steps. A two-step chromatographic process was then used to eliminate remaining impurities. Using this approach, recombinant human antibody expressed in tobacco could be purified to greater than 95% purity with 50% overall recovery (ca. 12.5 mg antibody/kg tobacco tissues).

► Efficient purification of transgenic monoclonal antibody. ► Use of cascade ultrafiltration as a pre-enrichment technique. ► 97% impurities removal and almost complete antibody recovery by ultrafiltration. ► Rational combination of ultrafiltration with chromatography. ► Greater than 95% purity with 50% overall recovery.

Keywords
Monoclonal antibody; Transgenic tobacco; Purification; Ultrafiltration; Chromatography
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Purification of transgenic tobacco-derived recombinant human monoclonal antibody
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 72, 15 March 2013, Pages 33–41
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us