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Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect™

Paper ID Volume ID Publish Year Pages File Format Full-Text
33702 44988 2009 5 PDF Available
Title
Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect™
Abstract

Recent years have seen tremendous progress in next generation sequencing technologies, allowing genomic sequencing in a highly cost-effective manner. However, sample preparation for these sequencers remains a bottleneck as the human genome is too complex to be routinely resequenced. We present here an in-depth study of HybSelect™, a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle.

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Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect™
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 26, Issue 5, 30 November 2009, Pages 229–233
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us