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Detection of protein–protein interactions by ribosome display and protein in situ immobilisation

Paper ID Volume ID Publish Year Pages File Format Full-Text
33811 44990 2009 5 PDF Available
Title
Detection of protein–protein interactions by ribosome display and protein in situ immobilisation
Abstract

We describe a method for identification of protein–protein interactions by combining two cell-free protein technologies, namely ribosome display and protein in situ immobilisation. The method requires only PCR fragments as the starting material, the target proteins being made through cell-free protein synthesis, either associated with their encoding mRNA as ribosome complexes or immobilised on a solid surface. The use of ribosome complexes allows identification of interacting protein partners from their attached coding mRNA. To demonstrate the procedures, we have employed the lymphocyte signalling proteins Vav1 and Grb2 and confirmed the interaction between Grb2 and the N-terminal SH3 domain of Vav1. The method has promise for library screening of pairwise protein interactions, down to the analytical level of individual domain or motif mapping.

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Detection of protein–protein interactions by ribosome display and protein in situ immobilisation
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 26, Issue 6, 31 December 2009, Pages 277–281
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us