fulltext.study @t Gmail

Yeast cultures with UCP1 uncoupling activity as a heating device

Paper ID Volume ID Publish Year Pages File Format Full-Text
33814 44990 2009 7 PDF Available
Title
Yeast cultures with UCP1 uncoupling activity as a heating device
Abstract

Uncoupling proteins (UCPs) are mitochondrial transporters that facilitate controlled dissipation of the proton gradient and thus regulate energetic efficiency. The heat generating capacity of UCP from brown adipose tissue was investigated in yeasts expressing the protein recombinantly under conditions in which the temperature of the growth medium was measured directly. A Liquid Culture Calorimeter (LCC) was built consisting of a thermally isolated culture flask able to keep yeast cultures warm without resorting to additional heating. The exact internal temperature of the cultures was monitored for 24 h through a thermocouple connected to a data logger. Under these conditions, significant temperature increases (1 °C) in the media were recorded when yeast strains expressing endogenously active UCP1 mutants were grown. This is the first direct evidence, in a eukaryotic microbial model, of a temperature rise associated with uncoupling activity, and could be seen as the first step toward developing a biological heating device.

First Page Preview
Yeast cultures with UCP1 uncoupling activity as a heating device
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 26, Issue 6, 31 December 2009, Pages 300–306
Authors
, , , , , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us