fulltext.study @t Gmail

PCR amplification and cloning of tyrosine decarboxylase involved in synephrine biosynthesis in Citrus

Paper ID Volume ID Publish Year Pages File Format Full-Text
33821 44992 2010 9 PDF Available
Title
PCR amplification and cloning of tyrosine decarboxylase involved in synephrine biosynthesis in Citrus
Abstract

BackgroundThe phenolic amine synephrine is a vascoconstrictor and bronchiectatic agent and holds promise as an aid to weight management and obesity reduction. Synephrine is structurally similar to the active ingredients of several commercial cold remedies. Some Citrus contain high concentrations of synephrine. An enzyme involved in synephrine biosynthesis, tyrosine decarboxylase (TYDC), is a pyridoxal-5′-phosphate (PLP)-dependent enzyme that decarboxylates tyrosine to yield CO2 and tyramine. We used PCR to screen, clone and sequence this gene from various synephrine producing and nonproducing Citrus species and varieties to determine if DNA sequence of this gene correlated with synephrine presence.ResultsPCR amplification and comparison of DNA sequence indicates DNA sequence differences that may cause production of truncated proteins to be produced in some nonsynephrine producing Citrus.ConclusionSynephrine production may be genetically determined in part by the gene for TYDC.

First Page Preview
PCR amplification and cloning of tyrosine decarboxylase involved in synephrine biosynthesis in Citrus
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 27, Issue 4, 30 September 2010, Pages 308–316
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us