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Engineering Escherichia coli to convert acetic acid to free fatty acids

Paper ID Volume ID Publish Year Pages File Format Full-Text
3383 167 2013 10 PDF Available
Title
Engineering Escherichia coli to convert acetic acid to free fatty acids
Abstract

•Overexpress acetate utilization and fatty acids biosynthesis pathways in Escherichia coli.•Optimize cultivation of engineered strain for fatty acids production using acetate.•Investigate acetate metabolism for biosynthesis in the engineered strain via 13C labeling.•Produce fatty acids using acetate from lignocellulosic hydrolysate or anaerobic digestion.

Fatty acids (FAs) are promising precursors of advanced biofuels. This study investigated conversion of acetic acid (HAc) to FAs by an engineered Escherichia coli strain. We combined established genetic engineering strategies including overexpression of acs and tesA genes, and knockout of fadE in E. coli BL21, resulting in the production of ~1 g/L FAs from acetic acid. The microbial conversion of HAc to FAs was achieved with ~20% of the theoretical yield. We cultured the engineered strain with HAc-rich liquid wastes, which yielded ~0.43 g/L FAs using waste streams from dilute acid hydrolysis of lignocellulosic biomass and ~0.17 g/L FAs using effluent from anaerobic-digested sewage sludge. 13C-isotopic experiments showed that the metabolism in our engineered strain had high carbon fluxes toward FAs synthesis and TCA cycle in a complex HAc medium. This proof-of-concept work demonstrates the possibility for coupling the waste treatment with the biosynthesis of advanced biofuel via genetically engineered microbial species.

Keywords
AccABCD, acetyl CoA carboxylase; Acs, acetyl CoA synthase; AckA, acetate kinase; FadD, acyl CoA synthetase; FadE, acyl CoA dehydrogenase; PoxB, pyruvate oxidase; Pta, phosphate acetyltransferase; TesA, acyl ACP thioesterase; FAs, free fatty acids; HAc, ac
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 76, 15 July 2013, Pages 60–69
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us