fulltext.study @t Gmail

Optimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium sp.

Paper ID Volume ID Publish Year Pages File Format Full-Text
33832 44992 2010 8 PDF Available
Title
Optimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium sp.
Abstract

Nitrogen (N) sources, the critical medium component, were optimized for squalene production by microalga Aurantiochytrium sp. in heterotrophic cultures. In screening experiments monosodium glutamate, yeast extract and tryptone were found to enhance cell growth and squalene production. The optimal levels of the three nitrogen sources were further determined through central composite experimental design. The squalene content and yield were both influenced not only by monosodium glutamate, tryptone and yeast extract, but also by their interactions. The squalene content and squalene yield were described by the second-order polynomial equations with high confidence levels (>99%). The optimal concentrations of monosodium glutamate, yeast extract and tryptone were predicted to be 6.61 g/L, 6.13 g/L and 4.50 g/L for squalene content and 6.94 g/L, 6.22 g/L and 4.40 g/L for squalene yield, respectively. In the verification experiment, the squalene content and squalene yield reached 0.72 mg/g and 5.90 mg/L, respectively, which were much higher than those obtained in previous studies.

First Page Preview
Optimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium sp.
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 27, Issue 4, 30 September 2010, Pages 382–389
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us