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Effect of high hydrostatic pressure on the enzyme activities in Saccharomyces cerevisiae and Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
33840 44992 2010 5 PDF Available
Title
Effect of high hydrostatic pressure on the enzyme activities in Saccharomyces cerevisiae and Escherichia coli
Abstract

The purpose of this study was to evaluate the effect of high hydrostatic pressure (HHP) on the enzyme activities in Saccharomyces cerevisiae (ATCC 16664) and Escherichia coli (ATCC 11229). Enzyme activities before and after HHP treatment were determined using an APIZYME® enzyme assay kit. Thirteen active enzymes were detected in S. cerevisiae and E. coli. Pressure treatment at 448 MPa for 30 s at 23 °C resulted in different effects on enzymes in S. cerevisiae and E. coli. HHP completely inactivated lipase, cystine arylamidase, and chymotrypsin and moderately inactivated esterase, esterase lipase, leucine arylamidase, valine arylamidase and α-glucosidase in S. cerevisiae. In E. coli, esterase, esterase lipase, lipase, valine arylamidase, cystine arylamidase, trypsin, α-glucosidase, and β-glucuronidase were completely inactivated and leucine arylamidase and β-galactosidase retained partial activities. Phosphoric hydrolases were not inactivated in both microorganisms. The use of the enzyme assay kit provided rapid and useful information on the microorganisms’ enzymes and their sensitivity to HHP treatment in a simple manner.

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Effect of high hydrostatic pressure on the enzyme activities in Saccharomyces cerevisiae and Escherichia coli
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 27, Issue 4, 30 September 2010, Pages 440–444
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering