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Study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from Aspergillus oryzae

Paper ID Volume ID Publish Year Pages File Format Full-Text
33884 44996 2011 5 PDF Available
Title
Study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from Aspergillus oryzae
Abstract

The study had mainly investigated the synthesis of sucrose-6-acetate (s-6-a) in fructosyltransferase action. The synthesis reaction of s-6-a was performed between sucrose and glucose-6-acetate (g-6-a), and identified by high performance liquid chromatography (HPLC). According to the reaction of s-6-a catalyzed by fructosyltransferase from Aspergillus oryzae, the effect factors of reaction, such as the ratio of g-6-a to sucrose, temperature, time, pH, substrate and enzyme concentration in the reaction, were investigated. All results indicated that the fructosyltransferase could catalyze the s-6-a synthesis, and the optimal conditions of fructosyltransferase in reaction were 50 °C, pH 6.2, 48 h reaction time, 60% sucrose, 1:3 ratio of g-6-a to sucrose and 4.0 mg/L concentration of enzyme. This study plays the important role in sucralose synthesis, because it is very cumbersome in the reported methods.

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Study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from Aspergillus oryzae
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 1, 31 January 2011, Pages 14–18
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Any Questions? feel free to contact us