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Improvement of xylanase production by thermophilic fungus Thermomyces lanuginosus SDYKY-1 using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
33888 44996 2011 7 PDF Available
Title
Improvement of xylanase production by thermophilic fungus Thermomyces lanuginosus SDYKY-1 using response surface methodology
Abstract

Response surface methodology (RSM) was employed to optimize medium composition for the production of a thermostable xylanase from T. lanuginosus SDYKY-1 using economical carbon and nitrogen sources (soybean meal and corncobs, respectively). Plackett–Burman (P–B) design was applied to evaluate the effects of nine variables (powdered corncobs, soybean meal, Tween-80, CaCl2, MgSO4·7H2O, FeSO4, KH2PO4, initial pH and inoculum culture volume). Corncobs, soybean meal and FeSO4 were found to significantly influence on the xylanase production. The concentrations of these three factors were therefore optimized using central composite design and RSM. Adjusting the concentration of corncobs to 38.7 g/L, soybean meal to 17.5 g/L and FeSO4 to 0.26 g/L favored maximum xylanase production. Xylanase activity of 3078 U/mL was obtained after optimization, which was a 144% increase that obtained before optimization (1264 U/mL).

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Improvement of xylanase production by thermophilic fungus Thermomyces lanuginosus SDYKY-1 using response surface methodology
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 1, 31 January 2011, Pages 40–46
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us