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Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestivum

Paper ID Volume ID Publish Year Pages File Format Full-Text
33903 44997 2009 9 PDF Available
Title
Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestivum
Abstract

Triticum aestivum is of major importance both nutritionally and economically. Introduction of new genes has been difficult to apply to elite wheat varieties mainly as a result of their recalcitrance to prerequisite tissue culture. We attempted to improve the frequency of wheat transformation by exposing plants to high level of ammonium nitrate. Our experiments showed that modification of the ammonium nitrate content in the direct somatic embryogenesis induction medium can increase the number of primary embryos produced over twofold in the elite hard red wheat cultivar Superb. The number of primary embryos that were capable of transitioning into shoot development also increased twofold. Biolistic transformation efficiency improved as much as sevenfold when targeted scutellar tissue was exposed to elevated ammonium nitrate levels. This simple approach could become extremely useful for increasing transformation efficiency in wheat.

Keywords
Triticum aestivum; Direct somatic embryogenesis; Ammonium nitrate; Biolistic transformation; Transformation efficiency; Transgenic plant
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Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestivum
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 26, Issues 1–2, 1 October 2009, Pages 44–52
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us