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Screening and characterization of a phosphopentomutase useful for enzymatic production of 2′-deoxyribonucleoside

Paper ID Volume ID Publish Year Pages File Format Full-Text
33907 44997 2009 8 PDF Available
Title
Screening and characterization of a phosphopentomutase useful for enzymatic production of 2′-deoxyribonucleoside
Abstract

Bacillus sphaericus AKU 229 was found to produce an acetaldehyde-tolerant and phosphorylated compound-tolerant phosphopentomutase useful for enzymatic 2′-deoxyribonucleoside production. The gene encoding the phosphopentomutase was cloned and expressed in Escherichia coli. The E. coli expressing B. sphaericus phosphopentomutase was an excellent catalyst as to production of 2′-deoxyribonucleoside in the presence of acetaldehyde and phosphorylated compounds such as fructose 1,6-diphosphate, and d-glyceraldehyde 3-phosphate, which are derived from glucose through glycolysis with yeast cells, and exist abundantly in the practical reaction mixture for enzymatic 2′-deoxyribonucleoside production.

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Screening and characterization of a phosphopentomutase useful for enzymatic production of 2′-deoxyribonucleoside
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 26, Issues 1–2, 1 October 2009, Pages 75–82
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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