fulltext.study @t Gmail

Optimization of medium using response surface methodology for l-DOPA production by Pseudomonas sp. SSA

Paper ID Volume ID Publish Year Pages File Format Full-Text
3399 168 2013 10 PDF Available
Title
Optimization of medium using response surface methodology for l-DOPA production by Pseudomonas sp. SSA
Abstract

•Optimization of nutritional parameters for bacterial l-DOPA production using Response surface methodology.•The Plackett–Burman design and Box–Behnken design was employed for optimization of media.•The maximum l-DOPA production obtained experimentally was found to be 3.251 ± 0.12 g l−1.

l-3,4-Dihydroxyphenylalanine (l-DOPA) is the most potent drug used for treatment of Parkinsonism. Statistical optimization of nutritional parameters for the production of l-DOPA by Pseudomonas sp. SSA has been carried out using response surface methodology (RSM). Four most significant medium constituents identified by initial screening method of Plackett–Burman (PB) were glucose, peptone, l-tyrosine and CuSO4. In order to investigate quantitative effects of the four variables selected from PB design on l-DOPA production, Box–Behnken design was subsequently employed for further optimization. The medium having glucose 1.69 g l−1, peptone 1.61 g l−1, l-tyrosine 4.11 g l−1 and CuSO4 0.03 g l−1 was found to be optimum for maximum l-DOPA production. The optimization strategies used lead to a 6.27-fold increase in l-DOPA yield (3.251 ± 0.12 g l−1) compared to non-optimized medium (0.572 ± 0.1 g l−1). l-DOPA produced was further characterized by spectroscopic techniques, such as HPTLC, HPLC and GC–MS.

Keywords
Pseudomonas sp. SSA; l-DOPA; Response surface methodology; Tyrosinase; HPTLC
First Page Preview
Optimization of medium using response surface methodology for l-DOPA production by Pseudomonas sp. SSA
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 74, 15 May 2013, Pages 36–45
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us