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The response of endothelial cells to polymer surface composed of nanometric micelles

Paper ID Volume ID Publish Year Pages File Format Full-Text
34087 45001 2009 9 PDF Available
Title
The response of endothelial cells to polymer surface composed of nanometric micelles
Abstract

The response of endothelial cells (ECs) to a polyurethane (PU) characteristic of surface micelles (∼89 nm in diameter) and two novel nanocomposites (PU–Au) and (PU–Ag) containing smaller surface micelles (∼14–22 nm in diameter) was investigated. The molecular mechanism by which ECs reacted to nanometric surface micelles was examined. On PU–Au and PU–Ag, cell migration rate was promoted. This was accompanied by the upregulation of endothelial nitric oxide synthase (eNOS) and phosphorylated-Akt (p-Akt) expression. The induced eNOS and p-Akt expression was inhibited by LY294002, indicating that the effect of nanocomposites could be attributed to the PI3K pathway. An elevation of intracellular calcium concentration was noted. Additionally, more actin fibers were induced by PU–Au and PU–Ag. Reduction of actin expression upon addition of Y-27632 (an inhibitor of Rho-GTPase) and SU-1498 (an inhibitor of VEGF-R2) was observed. It was concluded that the nanometric micelles on PU surface may interact with ECs and accelerate their migration by increasing cytoplasmic Ca2+ and stimulating the PI3K/Akt/eNOS signaling pathway.

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The response of endothelial cells to polymer surface composed of nanometric micelles
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 25, Issue 4, April 2009, Pages 235–243
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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