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Potential of the solid-state fermentation of soy fibre residues by native microbial populations for bench-scale alkaline protease production

Paper ID Volume ID Publish Year Pages File Format Full-Text
3411 168 2013 5 PDF Available
Title
Potential of the solid-state fermentation of soy fibre residues by native microbial populations for bench-scale alkaline protease production
Abstract

The production of alkaline proteases by solid-state fermentation (SSF) was evaluated. The effect of three agro-industrial residues was examined: coffee husk, hair waste from the tanning industry and soy fibre residues. Soy fibre presented the highest yield for protease production at the laboratory scale (37 °C, 100 g samples). Consequently, experiments with soy fibre (F) and soy fibre with 10% compost as an inoculum (FC) were performed for 14 days in 4.5 L bench-scale aerobic near-adiabatic reactors. The highest activity occurred under thermophilic conditions and a high respiration activity of 47,331 ± 1391 U/g dry matter for F and 18,750 ± 1596 U/g dry matter for FC, which are much higher values than those reported in other studies. Alkaline proteases showed maximum stability at pH 11 and temperatures of 43.8 °C (F) and 30 °C (FC), which was determined by a full factorial experimental design. Storage assays demonstrated that 90% of enzyme activity was preserved for three months by lyophilising or freezing the samples at −80 °C.

► Diverse organic wastes present high potential as substrates for protease production. ► Our findings consolidate solid-state fermentation as a waste valorisation process. ► Respiration indices are useful to determine the maximum enzyme activity. ► Soy fibre exhibits the highest potential as a source of protease. ► Alkaline pH and mesophilic temperature were optimal for protease activity.

Keywords
Solid-state fermentation; Alkaline protease; Respiration activity; Experimental design; Bench scale production
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Potential of the solid-state fermentation of soy fibre residues by native microbial populations for bench-scale alkaline protease production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 74, 15 May 2013, Pages 15–19
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us