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Global metabolite profiling of agarose degradation by Saccharophagus degradans 2-40

Paper ID Volume ID Publish Year Pages File Format Full-Text
34112 45003 2010 13 PDF Available
Title
Global metabolite profiling of agarose degradation by Saccharophagus degradans 2-40
Abstract

Saccharophagus degradans is a potent degrader of marine and plant cell wall polysaccharides. In particular, it is capable of degrading and metabolizing agarose that is the main component of marine red algae. To understand its degradation and metabolism of agarose along with the agarase expression profile, S. degradans was grown using different carbon sources including galactose, agarose, glucose and cellulose. The metabolite profiling was conducted by using GC–TOF MS and in-house programmed database, BinBase. When the metabolite profiles of cells on galactose and agarose were compared, principal component analysis of 133 identified metabolites revealed clear separations between the groups on galactose and agarose. S. degradans grown on agarose was found to use different carbon catabolic pathways from that grown on other carbon sources. The metabolite profile of cells grown using galactose had increased abundances of glycerol, glycerol derivatives and fatty acids. The use of polysaccharides such as agarose or cellulose led to the increased abundances of amino acids and intermediates of nucleotide biosynthesis.

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Global metabolite profiling of agarose degradation by Saccharophagus degradans 2-40
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 27, Issue 2, 31 May 2010, Pages 156–168
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us