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An improved water-soluble/stereospecific biotransformation of aporphine alkaloids in Stephania epigaea to 4R-hydroxyaporphine alkaloids by Clonostachys rogersoniana

Paper ID Volume ID Publish Year Pages File Format Full-Text
34138 45005 2016 8 PDF Available
Title
An improved water-soluble/stereospecific biotransformation of aporphine alkaloids in Stephania epigaea to 4R-hydroxyaporphine alkaloids by Clonostachys rogersoniana
Abstract

•Aporphine alkaloids in S. epigaea were transformed into 4R-hydroxyaporphine alkaloids.•The biotransformation occurred by fermentation with C. rogersoniana.•The biotransformation was validated by solid- and liquid-state fermentations.•The produced alkaloids were more water soluble than the original alkaloids.•Cytochrome P450 enzymes were confirmed to participate in the catalysis.

Aporphine alkaloids were transformed into the corresponding stereospecific 4R-hydroxyaporphine alkaloids through the solid-state fermentation of Stephania epigaea with Clonostachys rogersoniana. This process was validated by both solid- and liquid-state fermentations with aporphine alkaloids as substrates. Cytochrome P450 enzymes were confirmed to participate in the catalysis of this biotransformation. 4R-Hydroxyaporphine alkaloids exhibit the same levels of acetylcholinesterase (AChE) inhibitory and cytotoxic activities as aporphine alkaloids and are considerably more water soluble than aporphine alkaloids, indicating their potential as water-soluble AChE inhibitors and antitumor agents. This paper suggests that C. rogersoniana fermentation can facilitate a novel biotransformation of aporphine alkaloids in S. epigaea to 4R-hydroxyaporphine alkaloids.

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Keywords
Aporphine alkaloid; 4R-Hydroxyaporphine alkaloid; Stephania epigaea; Biotransformation; Clonostachys rogersoniana; Water solubility
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An improved water-soluble/stereospecific biotransformation of aporphine alkaloids in Stephania epigaea to 4R-hydroxyaporphine alkaloids by Clonostachys rogersoniana
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 51, Issue 8, August 2016, Pages 933–940
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us