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Precursor-feeding strategy on the triterpenoid production and anti-inflammatory activity of Antrodia cinnamomea

Paper ID Volume ID Publish Year Pages File Format Full-Text
34139 45005 2016 9 PDF Available
Title
Precursor-feeding strategy on the triterpenoid production and anti-inflammatory activity of Antrodia cinnamomea
Abstract

•Precursor-feeding strategy was used for production of triterpenoids in A. cinnamomea.•Squalene-feeding increased 4,7-D, De-sul, 15α, and De-eb contents.•Stigmasterol-feeding increased ZaA, 15α and De-eb contents.•A. cinnamomea exhibit anti-inflammation by inhibiting NO production in BV-2 cells.•A. cinnamomea inhibits significantly iNOS and COX-2 production.

Antrodia cinnamomea has commercially been used in the formulation of nutraceuticals and functional foods. A. cinnamomea was fed exogenous sterols including squalene, cholesterol, and stigmasterol to enhance its triterpenoid content. Four triterpenoids were identified in A. cinnamomea, namely dehydrosulphurenic acid (De-sul), zhankuic acid A (ZaA), 15α-acetyl-dehydrosulphurenic acid (15α) and dehydroeburicoic acid (De-eb), and one polyphenyl compound, 4,7-dimethoxy-5-methyl-1,3-benzodioxole (4,7-D). Maximum ZaA and 15α contents of 2.84 and 48.07 μg/mg dry weight, respectively were achieved by 100 μM squalene-feeding, and maximum De-sul and De-eb contents of 69.08 and 47.91 μg/mg dry weight were achieved by 10 and 100 μM stigmasterol-feeding, respectively. To study the anti-inflammatory potential of A. cinnamomea, lipopolysaccharide-induced nitric oxide (NO) production, NADPH oxidase (NOX), inducible NO synthetase (iNOS) and cyclooxygenase-2 (COX-2) expression in murine microglial cells, BV-2, were evaluated. ZaA and 50 μM squalene-feeding of A. cinnamomea inhibited LPS-induced NO, iNOS and COX-2 expression in BV-2 cells.

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Keywords
Antrodia cinnamomea; Triterpenoids; Precursor-feeding; Squalene; Anti-inflammation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 51, Issue 8, August 2016, Pages 941–949
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us