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Addition of abscisic acid increases the production of chitin deacetylase by Colletotrichum gloeosporioides in submerged culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
34141 45005 2016 8 PDF Available
Title
Addition of abscisic acid increases the production of chitin deacetylase by Colletotrichum gloeosporioides in submerged culture
Abstract

•Abscisic acid (ABA) exerted a positive and significant effect on enzyme production.•ABA accelerates the germination process of C. gloeosporioides and increases CDA production.•A significant increase in CDA activity was achieved with fed batch.•ABA changes acetylated fractions of chitin from fungal cell walls.

The activity of chitin deacetylase from Colletotrichum gloeosporioides was studied by the addition of phytohormones (gibberellic acid, indole acetic acid, and abscisic acid) and amino sugars (glucosamine and N-acetyl glucosamine) in culture media. Abscisic acid exerted a positive and significant effect on enzyme production with 9.5-fold higher activity (1.05 U mg protein−1) than the control (0.11 U mg protein−1). Subsequently, this phytohormone was used in batch culture with higher chitin deacetylase activity being found at acidic pH (3.5) than at neutral pH (7). Furthermore, the highest activity was determined at the acceleration growth phase. The chitin deacetylase production was ascribed to the lag phase within the spore germination process and germ tube elongation instead of during the formation of appressoria, as evidenced by the scanning electronic microscopy results. Therefore, more inoculum and medium containing abscisic acid were added to the fed-batch culture, resulting in a significant increase in chitin deacetylase activity (3.64 U mg protein−1). The addition of abscisic acid led to changes in the acetylation degree of chitin extracted from the cell wall of C. gloeosporioides, with lower degree of acetylation (DA of 75.6%) than that determined with the culture without abscisic acid (DA of 90.6%).

Graphical abstractAbscisic acid (ABA) accelerates germination process of Colletotrichum gloeosporioides and increases chitin deacetylases production.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Tween 80 (PubChem CID: 5281955); Glucose (PubChem CID: 5793); l-Glutamic acid (PubChem CID: 33032); Thiamine hydrochloride (PubChem CID: 6202); Nicotinic acid hydrochloride (PubChem CID: 71558); N-Acetyl-d-glucosamine (PubChem CID: 439174); d-Glucosamine
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Addition of abscisic acid increases the production of chitin deacetylase by Colletotrichum gloeosporioides in submerged culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 51, Issue 8, August 2016, Pages 959–966
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us