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High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism

Paper ID Volume ID Publish Year Pages File Format Full-Text
34142 45005 2016 6 PDF Available
Title
High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism
Abstract

•Xylose catabolism by Saccharomyces cerevisiae was inhibited by ethanol.•Xylose catabolism was resumed once ethanol was removed from the fermentation broth.•A fermentation-pervaporation process was used for in situ removal of ethanol.•High titer of ethanol was produced from corn stover hydrolysate.

Efficient xylose utilization is critical for the production of fuels from biomass hydrolysates. It is known that xylose catabolism is inhibited by glucose. In this study, we showed that ethanol also inhibits xylose catabolism. By introducing a xylose metabolic pathway into Saccharomyces cerevisiae and using evolutionary engineering, an engineered S. cerevisiae strain, W32N55, was obtained that can anaerobically ferment xylose to ethanol. The effect of ethanol on xylose utilization was investigated. The results showed that xylose catabolism was inhibited upon the addition of ethanol, and it resumed once ethanol was removed. Based on these results, a fermentation–pervaporation coupling process was developed. After the in situ removal of ethanol, 150 g/L glucose and 31 g/L xylose were consumed in 72 h, providing a total of 76 g/L ethanol and an overall total sugar yield of 0.42 g/g. We believe that this strain will be valuable to the bio-ethanol industry.

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Keywords
Cellulosic ethanol; Ethanol inhibition; Fermentation–pervaporation; Saccharomyces cerevisiae; Xylose catabolism
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High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 51, Issue 8, August 2016, Pages 967–972
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us