fulltext.study @t Gmail

Alleviation of proteolytic degradation of recombinant human bone morphogenetic protein-4 by repeated batch culture of Chinese hamster ovary cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
34154 45005 2016 7 PDF Available
Title
Alleviation of proteolytic degradation of recombinant human bone morphogenetic protein-4 by repeated batch culture of Chinese hamster ovary cells
Abstract

•CHO cells producing rhBMP-4 were cultivated at various temperature and pH.•A significant degradation of rhBMP-4 was observed in the decline phase of growth.•Increased proteolytic activity was in part responsible for the degradation of rhBMP-4.•The addition of protease inhibitors was not effective in enhancing rhBMP-4 production.•Repeated batch cultures have a potential as a means for improved rhBMP production.

The effects of culture temperature and pH on cell growth and recombinant human bone morphogenetic protein-4 (rhBMP-4) production were investigated to maximize rhBMP-4 production from Chinese hamster ovary cells. Cells were cultivated in a bioreactor at different culture pH levels (6.7, 6.9, 7.2, and 7.5) and temperatures (37 °C and 33 °C). The highest rhBMP-4 concentration (4.6 ± 0.5 μg/mL) was obtained at pH 7.2 and 37 °C. Regardless of culture temperature and pH, significant rhBMP-4 degradation via increased proteolytic activity was observed in the decline growth phase. Protease inhibitors such as EDTA and leupeptin were toxic and thus did not enhance rhBMP-4 production. High viability was achieved in repeated batch cultures with frequent medium exchanges. These cultures showed enhanced rhBMP-4 productivity, demonstrating the potential of repeated batch cultures as a means for improved rhBMP-4 production.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
BMP-4; CHO cells; Culture temperature; Culture pH; Proteolytic degradation; Repeated batch cultures
First Page Preview
Alleviation of proteolytic degradation of recombinant human bone morphogenetic protein-4 by repeated batch culture of Chinese hamster ovary cells
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 51, Issue 8, August 2016, Pages 1078–1084
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us