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Condensed tannins from Ficus altissima leaves: Structural, antioxidant, and antityrosinase properties

Paper ID Volume ID Publish Year Pages File Format Full-Text
34156 45005 2016 8 PDF Available
Title
Condensed tannins from Ficus altissima leaves: Structural, antioxidant, and antityrosinase properties
Abstract

•The total phenolic content of Ficus altissima leaves was determined.•The concentration of extractable and bound condensed tannins was measured.•The structure was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.•Reversed-phase high-performance liquid chromatography electrospray ionization mass spectrometry was also used.•Condensed tannins exhibited remarkable antioxidant and antityrosinase activity.

The total phenolic content and the concentration of extractable and bound condensed tannins of Ficus altissima leaves were determined. The antioxidant activity of crude extracts and condensed tannins of F. altissima leaves was evaluated using 2,2-diphenyl-1-pichydrazyl (DPpH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP) methods. The results showed that condensed tannins possessed higher free radical scavenging power. The structure of condensed tannins was characterized using high-performance liquid chromatography electrospray ionization mass spectrometry coupled with thiolysis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The findings showed that condensed tannins from F. altissima leaves were mixtures of procyanidins, prodelphinidins, and propelargonidins with a degree of polymerization up to 30-mer. In addition, condensed tannins effectively protected plasmid DNA against free radical damage and alleviated t-butylhydroperoxide-induced cytotoxicity on human hepatocyte LO2 cells. Condensed tannins exhibited remarkable inhibitory effects on both monophenolase and diphenolase activities of tyrosinase. The IC50 values were estimated to be 256.7 ± 0.3 and 41.3 ± 0.2 μg/mL, respectively. For the diphenolase activity, inhibition by condensed tannins was reversible and of mixed-type. Condensed tannins from leaves of F. altissima were indicated to possess significant antioxidant and antityrosinase activities, suggesting that F. altissima might be a good source of proanthocyanidins with biological activity.

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Keywords
Condensed tannins; Ficus altissima; Antioxidant; Antityrosinase; Structural characterization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 51, Issue 8, August 2016, Pages 1092–1099
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us