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Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana

Paper ID Volume ID Publish Year Pages File Format Full-Text
3417 170 2013 6 PDF Available
Title
Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana
Abstract

This study evaluated the feasibility of transgenic Arabidopsis engineered to express the bacterial heavy metal transporter MerC for the phytoremediation of mercury pollution. MerC, MerC–SYP121, or MerC–AtVAM3 proteins were found to be expressed in leaf segments of transgenic plants using an anti-MerC antibody immunostaining method. By sucrose density gradient centrifugation and immunoblotting analyses, MerC, MerC–SYP121, and MerC–AtVAM3 were found to localized in the Golgi apparatus, plasma membrane, and vacuole membrane, respectively. Transgenic Arabidopsis plants that expressed merC–SYP121 were more resistant to mercury and accumulated significantly more of this metal than wild-type Arabidopsis. These results demonstrated that expression of the bacterial heavy metal transporter MerC promoted the transport and accumulation of mercury in transgenic Arabidopsis, which may be a useful method for improving plants for the phytoremediation of mercury pollution.

► This study showed that transgenic MerC-SNARE plants may be useful for mercury phytoremediation. ► MerC is a bacterial heavy metal transporter and SNARE is used as an organelle-targeting marker to direct MerC to specific membranes. ► Transgenic plants were more resistant to mercury and accumulated more mercury. ► MerC can promote the transport and accumulation of mercury in transgenic plants.

Keywords
AtVAM3; Bacterial heavy metal transport; MerC; Mercury; Phytoremediation; SYP121
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Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 71, 15 February 2013, Pages 19–24
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us