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Novel biocatalysts for glycerol conversion into 2,3-butanediol

Paper ID Volume ID Publish Year Pages File Format Full-Text
34181 45008 2016 9 PDF Available
Title
Novel biocatalysts for glycerol conversion into 2,3-butanediol
Abstract

•Conversion of pure and raw glycerol to 2,3-butanediol with safe strains is studied.•Screening of 850 isolated strains from biodiesel factories yielded two candidates.•Batch bioprocesses optimization with the Raoultella candidates was undertaken.•Taguchi strategy for experimental design and result comparison was employed.•R. terrigena and R. planticolla strains proved to be efficient.

Bioconversion of biodiesel-derived waste glycerol into high-value products is proposed as a solution to improve economic viability of biorefineries. Several microorganisms are able to metabolize glycerol into 2,3-butanediol (2,3-BD), a promising bulk chemical with wide applications: solvent, fuel additive, and feedstock for synthetic rubber production, among them. In the present work, a wide screening of microorganisms present both into the waste water treatment system in a biodiesel industry and in culture collections was carried out in order to evaluate their potential as new 2,3-BD producer biocatalysts. Two microorganisms for 2,3-BD production from glycerol as sole carbon source have been selected, namely Raoultella planticola CECT 843 and Raoultella terrigena CECT 4519. Raoultella strains belong to the non‐pathogenic bacteria class (biosafety level 1). This genus has not been previously described as biocatalyst for the studied process. The influence of operational temperature, organic acid addition, and yeast extract concentration on 2,3-BD yield and productivity have been studied through Taguchi design methodology as well as initial glycerol concentration. Based on these results, the feasibility of the process employing pure glycerol and different samples of raw glycerol has been demonstrated.

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Keywords
Biorefinery; Raw glycerol; 2,3-Butanediol; Raoultella terrigena; Raoultella planticola
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Novel biocatalysts for glycerol conversion into 2,3-butanediol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 51, Issue 6, June 2016, Pages 740–748
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us