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Single-step purification of ovalbumin from egg white using aqueous biphasic systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
34186 45008 2016 11 PDF Available
Title
Single-step purification of ovalbumin from egg white using aqueous biphasic systems
Abstract

•ABS composed of PEG and potassium citrate/citric acid were investigated.•Ovalbumin preferentially migrates to the PEG-rich phase.•Extraction efficiencies up to 98.9% were attained using commercial and pure ovalbumin.•The isolation of pure ovalbumin with a recovery yield of 65% was attained in a single-step.

The ability of aqueous biphasic systems (ABS) composed of polyethylene glycols of different molecular weights (PEG 400, 600 and 1000) and buffered aqueous solutions of potassium citrate/citric acid (pH 5.0–8.0) to selectively extract ovalbumin from egg white was here investigated. Phase diagrams, tie-lines and tie-line lengths were determined at 25 °C and the partitioning of ovalbumin in these systems was then evaluated. Aiming at optimizing the selective extraction of ovalbumin in the studied ABS, factors such as pH, PEG molecular weight and amount of the phase-forming components were initially investigated with pure commercial ovalbumin. In all ABS, it was observed a preferential partitioning of ovalbumin to the polymer-rich phase, with extraction efficiencies higher than 90%. The best ABS were then applied in the purification of ovalbumin from the real egg white matrix. In order to ascertain on the ovalbumin purity and yield, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and size exclusion high performance liquid chromatography (SE-HPLC) analyses were conducted, confirming that the isolation/purification of ovalbumin from egg white was completely achieved in a single-step with a recovery yield of 65%. The results obtained show that polymer-salt-based ABS allow the selective extraction of ovalbumin from egg white with a simpler approach and better performance than previously reported. Finally, it is shown that ovalbumin can be completely recovered from the PEG-rich phase by an induced precipitation using an inexpensive and sustainable separation platform which can be easily applied on an industrial scale.

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Keywords
Ovalbumin; Protein recovery; Separation; Aqueous two phase; Downstream processing; Egg white
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Single-step purification of ovalbumin from egg white using aqueous biphasic systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 51, Issue 6, June 2016, Pages 781–791
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us