fulltext.study @t Gmail

Exploiting the biological roles of the trypsin inhibitor from Inga vera seeds: A multifunctional Kunitz inhibitor

Paper ID Volume ID Publish Year Pages File Format Full-Text
34187 45008 2016 12 PDF Available
Title
Exploiting the biological roles of the trypsin inhibitor from Inga vera seeds: A multifunctional Kunitz inhibitor
Abstract

•Inga vera trypsin inhibitor (IVTI) is a Kunitz-type inhibitor composed of a single 20 kDa polypeptide chain.•IVTI inhibits the trypsin-like enzymes from several lepidopteran pests.•IVTI impairs the weight gain, survival and larval cycle of Anagasta kuehniella.•IVTI is a fungicidal to Candida buinensis.•IVTI prevents the proliferation of colorectal adenocarcinoma cells (CACO-2).

Here, the purification, biochemical and biological properties of a trypsin inhibitor from Inga vera seeds (IVTI) are described. Partial amino acid sequence of IVTI showed that it belongs to the MEROPS I03 Kunitz inhibitor family. Moreover, it is composed of a single 20 kDa polypeptide chain with one disulfide bridge and was capable of inhibiting bovine trypsin at a 1:1 molar ratio with a 1.15 nM inhibition constant. IVTI was stable over a wide range of temperature, pH and concentration of a reducing agent. IVTI also inhibited the trypsin-like enzymes from the midgut of lepidopteran pests, such as Anagasta kuehniella (89%), Spodoptera frugiperda (83%), Corcyra cephalonica (80%), Heliothis virescens (70%) and Helicoverpa zea (64%). Furthermore, bioinsecticidal assays against A. kuehniella demonstrated that IVTI affected larval development by impairing weight gain and survival, as well as altering the duration of the larval cycle. IVTI was also fungicidal to Candida buinensis and bacteriostatic agent to Escherichia coli. Further assays revealed that IVTI is a chemopreventive agent against human epithelial colorectal adenocarcinoma cells (CACO-2), reducing cell viability by 70% at 200 μg mL−1. In summary, these results demonstrate the multifaceted potential of IVTI as a biotechnological tool for agriculture and healthcare.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Inga vera; Plant-defense; Kunitz inhibitor; Antimicrobial; Anticancer; Bioinsecticidal
First Page Preview
Exploiting the biological roles of the trypsin inhibitor from Inga vera seeds: A multifunctional Kunitz inhibitor
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 51, Issue 6, June 2016, Pages 792–803
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us