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The effect of redox environment on l-lactic acid production by Lactobacillus paracasei—A proof by genetically encoded in vivo NADH biosensor

Paper ID Volume ID Publish Year Pages File Format Full-Text
34194 45010 2015 6 PDF Available
Title
The effect of redox environment on l-lactic acid production by Lactobacillus paracasei—A proof by genetically encoded in vivo NADH biosensor
Abstract

•Specific NADH biosensor (Frex) was introduced into lactic acid bacteria.•Frex was adopted for lactic acid production from the standpoint of process engineering.•Extracellular ORP level significantly influenced intracellular redox condition.•Cell growth and metabolism were dependent on different optimal ORP levels.

In this study, a reduced nicotinamide adenine dinucleotide hydride (NADH) biosensor (Frex) was introduced into lactic acid bacteria for the first time. By detecting the changes of NADH with environmental perturbations through NADH specificity, glucose metabolism, electron transfer chain inhibition, and pyruvate regulation, the feasible application of Frex in Lactobacillus paracasei was verified. Furthermore, the physiological and metabolic characteristics of the recombinant strain exhibited good performances on cell growth and metabolism, as well as the yield of l-lactic acid. Simultaneously, the level of oxidative and reductive potential (ORP) could be adopted to characterize the evolution of fermentation. Finally, from the viewpoint of process engineering with growing and resting cells, the in vivo measurement results using Frex revealed that intracellular redox conditions would be significantly influenced by extracellular ORP environments, as well as cell growth and metabolism were dependent on different optimal ORP levels in L. paracasei.

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Keywords
NADH; Biosensor; Lactic acid; ORP
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The effect of redox environment on l-lactic acid production by Lactobacillus paracasei—A proof by genetically encoded in vivo NADH biosensor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 12, December 2015, Pages 2029–2034
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us