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Engineering Escherichia coli for the production of adipic acid through the reversed β-oxidation pathway

Paper ID Volume ID Publish Year Pages File Format Full-Text
34198 45010 2015 6 PDF Available
Title
Engineering Escherichia coli for the production of adipic acid through the reversed β-oxidation pathway
Abstract

•A synthetic pathway for producing adipic acid in E. coli is proposed.•Each step of the pathway was confirmed in vitro.•Adipic acid production by the engineered E. coli was demonstrated.

The current study aimed to construct a functionally reversed β-oxidation cycle in Escherichia coli for the production of adipic acid, one of the most important dicarboxylic acids. Starting from the condensation of acetyl-CoA and succinyl-CoA, a synthetic pathway mimicking a reversed β-oxidation cycle was constructed in E. coli to produce adipyl-CoA, consisting of four enzymatic steps. Products of the synthetic pathway were confirmed by liquid chromatography-tandem mass spectrometry. To convert adipyl-CoA into adipic acid, a putative adipyl-CoA thioesterase (encoded by Acot8 from Mus musculus) was selected, and its functional expression in E. coli was confirmed by gas chromatography-mass spectrometry. Finally, when the reversed β-oxidation cycle and Acot8 were co-expressed in E. coli, the resulting strain produced 12 μg/L of adipic acid from glucose. These results suggest that the synthetic adipic acid pathway functions in E. coli but requires further optimization for increased production of adipic acid.

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Keywords
Adipic acid; Dicarboxylic acid; Acetyl-CoA; Succinyl-CoA; β-Oxidation cycle; Acot8
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Engineering Escherichia coli for the production of adipic acid through the reversed β-oxidation pathway
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 12, December 2015, Pages 2066–2071
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us