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Antioxidative effect of recombinant ice-binding protein (rLeIBP) from Arctic yeast Glaciozyma sp. on lipid peroxidation of Korean beef

Paper ID Volume ID Publish Year Pages File Format Full-Text
34202 45010 2015 6 PDF Available
Title
Antioxidative effect of recombinant ice-binding protein (rLeIBP) from Arctic yeast Glaciozyma sp. on lipid peroxidation of Korean beef
Abstract

•Recombinant ice-binding protein (rLeIBP) was cloned and expressed from the Arctic yeast Leucosporium sp.•rLeIBP markedly reduced thiobarbituric acid reactive substances (TBARS) value of Korean beef during cold storage.•rLeIBP improved antioxidative enzyme activities of Korean beef during cold storage.•rLeIBP treatment may be prolonged the shelf-life of Korean beef by delaying lipid peroxidation and microbial growth rates during cold storage.

In the present study, we showed that recombinant ice-binding protein (rLeIBP) from Arctic yeast Glaciozyma sp. prolonged the shelf-life of Korean beef by delaying lipid peroxidation and microbial growth rates during cold storage when it was embrocated in Korean beef before freezing. In addition, glutathione (GSH) and the antioxidant enzyme activities including catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPX) and superoxide dismutase (SOD) were higher in the rLeIBP treated Korean beef samples than the control group containing no rLeIBP. These results indicate that the rLeIBP may be used to treat beef prior to refrigeration, since rLeIBP was shown to retard microbial growth rates as well as lipid peroxidation and improve the antioxidant enzyme activities of beef during cold storage.

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Keywords
Recombinant ice-binding protein; Korean beef; Antioxidative enzyme activity; Thiobarbituric acid; Lipid peroxidation
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Antioxidative effect of recombinant ice-binding protein (rLeIBP) from Arctic yeast Glaciozyma sp. on lipid peroxidation of Korean beef
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 12, December 2015, Pages 2099–2104
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us