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Efficiency of a SDG-β-glucosidase from Bacillus altitudinis HK02 for the deglycation of glycosides from flaxseeds

Paper ID Volume ID Publish Year Pages File Format Full-Text
34213 45010 2015 6 PDF Available
Title
Efficiency of a SDG-β-glucosidase from Bacillus altitudinis HK02 for the deglycation of glycosides from flaxseeds
Abstract

•A SDG-β-glucosidase from B. altitudinis deglycates flaxseed glucosides.•The efficiency of hydrolyzation was based on the numbers of glucose moieties.•The enzyme activity could be enhanced by defatted flaxseed flour or inhibited by glucose.•It might be an inducible enzyme regulated by varieties of inducers or catabolites.

A novel secoisolariciresinol diglucoside (SDG)-β-glucosidase from Bacillus altitudinis HK02 is capable of deglycating phenolic glycosides from flaxseed. Three glycosides, SDG, coumaric acid glucoside (CAF) and ferulic acid glucoside (FAG), were purified from flaxseed and deglycated into aglycones by the prepared glycosidase. The deglycation rates of these phenolic glycosides were dependent on the number of sugar moieties on the glycones. The deglycation of SDG with two glucose molecules into secoisolariciresinol (SECO) occurred after both CAG and FAG were completely consumed. The efficiency of the enzymatic deglycation of these three glycosides could reach approximately 100%. The addition of defatted flaxseed flour or alkali–ethanol extract to the media enhanced enzyme activities and accelerated the conversion rate of SDG; however, carbon sources such as glucose, sucrose, fructose and mannitol significantly inhibited enzyme activities. The novel SDG-β-glucosidase was proposed as an inducible enzyme regulated by a variety of inducers or catabolites.

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Keywords
SDG; β-glucosidase; Bacillus altitudinis; Deglycosylation
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Efficiency of a SDG-β-glucosidase from Bacillus altitudinis HK02 for the deglycation of glycosides from flaxseeds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 12, December 2015, Pages 2188–2193
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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