fulltext.study @t Gmail

Kinetic study of solid phase demineralization by weak acids in one-step enzymatic bio-refinery of shrimp cuticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
34216 45010 2015 9 PDF Available
Title
Kinetic study of solid phase demineralization by weak acids in one-step enzymatic bio-refinery of shrimp cuticles
Abstract

•Formic acid met the initial aim of 99% demineralization yield and 95% deproteinization yield at a pH close to 3.5 and a molar ratio of 1.5.•The proposed one-step process proved efficient.•An effective empirical model of demineralization kinetics was defined.

We describe a one-step bio-refinery process for shrimp composites by-products. Its originality lies in a simple rapid (6 h) biotechnological cuticle fragmentation process that recovers all major compounds (chitins, peptides and minerals in particular calcium). The process consists of a controlled exogenous enzymatic proteolysis in a food-grade acidic medium allowing chitin purification (solid phase), and recovery of peptides and minerals (liquid phase). At a pH of between 3.5 and 4, protease activity is effective, and peptides are preserved. Solid phase demineralization kinetics were followed for phosphoric, hydrochloric, acetic, formic and citric acids with pKa ranging from 2.1 to 4.76. Formic acid met the initial aim of (i) 99% of demineralization yield and (ii) 95% deproteinization yield at a pH close to 3.5 and a molar ratio of 1.5. The proposed one-step process is proven to be efficient. To formalize the necessary elements for the future optimization of the process, two models to predict shell demineralization kinetics were studied, one based on simplified physical considerations and a second empirical one. The first model did not accurately describe the kinetics for times exceeding 30 min, the empirical one performed adequately.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Bio-refinery; Acidic enzymatic proteolysis; Shrimp cuticles; Demineralization kinetics and modeling
First Page Preview
Kinetic study of solid phase demineralization by weak acids in one-step enzymatic bio-refinery of shrimp cuticles
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 12, December 2015, Pages 2215–2223
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us