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Immobilization of carbonic anhydrase on epoxy-functionalized magnetic polymer microspheres for CO2 capture

Paper ID Volume ID Publish Year Pages File Format Full-Text
34218 45010 2015 8 PDF Available
Title
Immobilization of carbonic anhydrase on epoxy-functionalized magnetic polymer microspheres for CO2 capture
Abstract

•Epoxy-functionalized magnetic polymer microspheres were synthesized for CA immobilization.•The immobilized CA presented a higher stability and reusability than free CA.•The immobilized CA had a high magnetism and a good CO2 capture capacity.

Carbonic anhydrase (CA) is the most efficient catalyst for CO2 hydration and dehydration. In the present work, magnetic polymer microspheres functionalized with epoxy group were prepared, and CA enzyme was immobilized on the carries by selective covalent binding. The parameters affecting CA immobilization, such as pH, temperature and enzyme dose were investigated. Based on the investigation and the characterization of the microspheres, the kinetic parameters of the immobilized and the free CA were also evaluated. The value of the Michaelis–Menten constant (Km) and the maximum velocity (Vmax) of the immobilized CA was 8.077 mmol/L (mM) and 0.027 μmol/(min mL), while that of the free CA was 6.091 mM and 0.091 μmol/(min mL), respectively. Moreover, the performance of the thermal stability, storage stability and reusability of the immobilized CA confirmed that CA immobilized on the epoxy-functionalized magnetic polymer microspheres owned a stable and efficient catalytic ability on CO2 hydration, which seemed to be a suitable candidate for CO2 capture.

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Keywords
Carbonic anhydrase; Magnetic microspheres; Immobilization; Carbon capture
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Immobilization of carbonic anhydrase on epoxy-functionalized magnetic polymer microspheres for CO2 capture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 12, December 2015, Pages 2234–2241
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us