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Unstructured models for suspension cultures of Taxus media cells in a bioreactor under substrate-sufficient conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
3422 170 2013 10 PDF Available
Title
Unstructured models for suspension cultures of Taxus media cells in a bioreactor under substrate-sufficient conditions
Abstract

For a better understanding of the simulation, optimization, and process control in cell cultures, good kinetic models are necessary for large scale plant cell culture. In this paper, the systematic kinetics of taxol production by Taxus media cell suspension cultures in a stirred 15-L bioreactor under substrate-sufficient conditions and the absence of inducer intervention were studied. A kinetic model of cell growth was established by logistic equation, and kinetic unstructured models of substrate consumption, product synthesis and rheological behavior were constituted, which incorporated energy spilling. These models were verified by comparing the simulation results with those obtained experimentally. These results showed that energy spilling was a key factor that must be considered in constructing unstructured kinetic models of Taxus media cell suspension cultures in a stirred bioreactor under substrate-sufficient conditions. Besides, an optimized operation measure of decreasing energy spilling was proposed. An increase of 17.64% in cell biomass and 14.88% in taxol concentration were obtained when the strategy of limiting added carbon several times was experimentally implemented in a 15-L bioreactor. Results demonstrated that these established models should be helpful in the process prediction and operation optimization to guide the production and amplification of Taxus media cell suspension cultures in a bioreactor.

► We study Taxus cell cultures in a bioreactor under substrate-sufficient conditions. ► We study the systematic kinetics of Taxus cell cultures in a bioreactor. ► We constitute kinetic unstructured models which incorporated energy spilling. ► Models include models of cell growth, biochemical reactions and rheology. ► We propose an optimized operation strategy of decreasing energy spilling.

Keywords
Taxus media; Bioreactors; Large scale cultivation; Modeling; Kinetic parameters; Energy spilling
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Unstructured models for suspension cultures of Taxus media cells in a bioreactor under substrate-sufficient conditions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 71, 15 February 2013, Pages 62–71
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us