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Evaluation of OPS-agarose pseudo-affinity adsorption IgG2a mouse monoclonal antibody

Paper ID Volume ID Publish Year Pages File Format Full-Text
34222 45010 2015 8 PDF Available
Title
Evaluation of OPS-agarose pseudo-affinity adsorption IgG2a mouse monoclonal antibody
Abstract

•OPS immobilized on agarose-gel as ligand for purification of IgG2a monoclonal antibody.•OPS-agarose selectivity was affected by the nature of the buffer system and NaCl.•Pseudo-first-order kinetic model described the kinetic rate of IgG2a in OPS-agarose.

The amino acid ortho-phosphoserine (OPS) is a feasible alternative to expensive protein ligands for the purification of IgG due to its selectivity, elution under non-denaturing conditions, and low cost. In this study we investigated the adsorption of IgG2a mouse monoclonal antibody (MAb) from precipitate solution of hybridoma culture supernatant onto OPS-agarose adsorbent. The effects of spacer arm, buffer system, pH, and NaCl concentration on the adsorption of IgG2a MAb were evaluated. Screening buffer systems showed that Tris–HCl at pH 7.0 provided the highest IgG2a MAb yield (77%) and purification factor (31.8). The adsorption of IgG2a MAb was observed to decrease by approximately 90% as the NaCl concentration was increased from 0.0 to 100 mmol L−1. A kinetic study of IgG2a MAb adsorption showed that these data were in agreement with the pseudo-first-order kinetic model. This study suggests the potential for use of OPS-agarose in the purification of IgG2a MAb from hybridoma culture supernatants.

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Keywords
Monoclonal antibodies (MAb); IgG2a; Purification; Adsorption; ortho-Phosphoserine
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Evaluation of OPS-agarose pseudo-affinity adsorption IgG2a mouse monoclonal antibody
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 12, December 2015, Pages 2267–2274
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us