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Doxorubicin functionalized gold nanoparticles: Characterization and activity against human cancer cell lines

Paper ID Volume ID Publish Year Pages File Format Full-Text
34226 45010 2015 9 PDF Available
Title
Doxorubicin functionalized gold nanoparticles: Characterization and activity against human cancer cell lines
Abstract

•Loading of doxorubicin onto GNPs by a simple incubation method is reported.•The effect of doxorubicin-loaded GNPs against human cancer cell lines is reported.•GNPs can be successfully explored as an inert carrier for targeting cancer.

The aim of the study was to synthesize doxorubicin (DOX)-functionalized gold nanoparticles (GNPs) by a green method and to evaluate their anticancer potential against human cancer cell lines. These GNPs were synthesized with a green chemistry method and characterized by ultraviolet (UV) spectrophotometry, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zetasizer measurements. Surface plasmon resonance studies showed a clear UV-Visible peak at 532 nm, suggesting the formation of GNPs. FT-IR and XRD were used to determine the surface characteristics (presence of phytoconstituents) and crystalline nature of GNPs, respectively. The TEM and Zetasizer studies revealed a particle size of 74.7 ± 2.47 nm with a zeta potential of −19.13 ± 0.2. The synthesized GNPs were loaded with DOX by simple incubation method and evaluated for particle size, zeta potential, FT-IR and XRD to confirm drug loading. An in vitro anticancer assay of DOX-loaded GNPs (D-GNPs) against human cancer cell lines showed variations in responsiveness to D-GNPs, with significant activity against breast, lung, and prostate cancer cell lines. However, no significant difference was found in the percent cell viability of cervical, liver, and pancreatic cancer cell lines between DOX and D-GNPs. The results of the in vitro anticancer assay of D-GNPs against human cancer cell lines supports their potential for in vivo applications in cancer treatments.

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Keywords
Gold nanoparticles; Green synthesis; Doxorubicin; Cytotoxicity; MTT assay; Cancer cell lines
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Doxorubicin functionalized gold nanoparticles: Characterization and activity against human cancer cell lines
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 12, December 2015, Pages 2298–2306
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us