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Anti-adipogenic effect of oat hull extract containing tricin on 3T3-L1 adipocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
34228 45010 2015 8 PDF Available
Title
Anti-adipogenic effect of oat hull extract containing tricin on 3T3-L1 adipocytes
Abstract

•Oat hull extract showed significant anti-adipogenic activity in 3T3-L1 cells.•Tricin was identified as a major active compound in the extract.•Expression of adipocyte specific transcription factor genes was analyzed.

Oat hull ethyl acetate extract (50 μg/μL) significantly inhibited triglyceride accumulation in 3T3-L1 adipocytes without causing cytotoxicity. The major active compound in the extract was isolated and identified as 5,7,4′-trihydroxy-3′,5′-dimethoxyflavone, tricin by mass spectrometry and 1H and 13C NMR analyses. The tricin content in oat hull was approximately 18 mg/kg dry weight, and tricin constituted 9.6% of the total phenolic compounds in oat hull extract. The treatment of 3T3-L1 cells either with extract or purified tricin resulted in decreased lipid accumulation. The extract significantly decreased the activity of glycerol-3-phosphate dyhydrogenase, a marker of adipocyte differentiation in a dose dependent manner. The expression of major transcription factors, such as peroxisome proliferator-activated receptor (PPAR)-γ, CCATT/enhancer binding protein-α (CEBP/α), sterol regulatory binding protein 1 (SREBP1) and fatty acid synthase (FAS) was also significantly suppressed by the extract. When commercial purified tricin was added to 3T3-L1 cells the expression of all transcription factors were significantly decreased as low as 1.5 μg/μL treatment. These results suggest that oat hull extract containing tricin possesses anti-adipogeneic effect.

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Keywords
Oat hull extract; 3T3-L1 adipocytes; Tricin; Adipogenesis; Transcription factors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 12, December 2015, Pages 2314–2321
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us