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An o-aminobenzoic acid film-based immunoelectrode for detection of the cardiac troponin T in human serum

Paper ID Volume ID Publish Year Pages File Format Full-Text
3426 170 2013 8 PDF Available
Title
An o-aminobenzoic acid film-based immunoelectrode for detection of the cardiac troponin T in human serum
Abstract

An amperometric immunosensor for cardiac troponin T detection in human serum troponin T, a marker considered as “gold standard” for acute myocardial infarction diagnosis, is described. A stable carboxylic film to covalently bind antibodies against cTnT onto electrode surface was achieved with electropolymerization of the o-aminobenzoic acid. A fractional factorial study was performed to optimize the electropolymerization parameters. Cyclic voltammetry assays were carried out for characterize steps of the modified electrode surface. The obtained calibration curve at −0.05 V by amperometry presented a good linear response range from 0.05 to 5.0 ng mL−1 cTnT with a correlation coefficient of 0.992 (n = 6) and 0.016 ng mL−1 detection limit. The electrodes showed a good stability upon the analytical responses retaining 91.6% of its initial response after 18 days. This sensor showed outgoing results regarding sensitivity allowing reliable measurements of the cTnT at levels of clinical significance for acute myocardial infarctions diagnosis.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► An immunosensor based on polymer film was developed for cardiac troponin T (cTnT). ► Anti-cTnT was immobilized on o-aminobenzoic acid film. ► A detection limit of 16.0 pg mL−1 troponin T was achieved. ► It was analyzed the troponin T in human serum.

Keywords
Immunosensor; Poly(o-aminobenzoic acid); Acute myocardial infarction; Cardiac troponin T
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An o-aminobenzoic acid film-based immunoelectrode for detection of the cardiac troponin T in human serum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 71, 15 February 2013, Pages 97–104
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us