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A two-stage process for succinate production using genetically engineered Corynebacterium acetoacidophilum

Paper ID Volume ID Publish Year Pages File Format Full-Text
34266 45016 2015 9 PDF Available
Title
A two-stage process for succinate production using genetically engineered Corynebacterium acetoacidophilum
Abstract

•C. acetoacidophilum was first genetically engineered for succinate production.•A two-stage bioprocess using strain C. acetoacidophilum ΔldhA/pXMJ19-pyc was developed.•The succinate concentration reached 908 mM with a yield of 1.34 mol (mol glucose)−1.

Corynebacterium acetoacidophilum, which is related to Corynebacterium glutamicum, was genetically engineered to produce succinate for the first time. The results of genetic experiments in C. acetoacidophilum ATCC13870 showed that disrupting the lactate and acetate formation pathways, as well as overexpressing the anaplerotic enzyme pyruvate carboxylase, could increase succinate production and decrease the formation of by-products. Among the six engineered C. acetoacidophilum strains, ΔldhA, ΔldhAΔpta-ackA, ΔldhAΔpta-ackAΔctfA, ΔldhAΔaceE, ΔldhA/pXMJ19-pyc, and ΔldhAΔaceE/pXMJ19-pyc, merely deleting the l-lactate dehydrogenase gene ldhA and overexpressing the pyruvate carboxylase gene pyc yielded the optimum production of succinate under oxygen deprivation. A two-stage bioprocess was conducted in a 3-L fermenter to produce succinate using strain ΔldhA/pXMJ19-pyc, in which cells were first grown aerobically, after which glucose and bicarbonate were directly added to the cultures for conversion under oxygen-deprived conditions. The succinate concentration reached 908 mM, with an overall yield of 1.34 mol (mol glucose)−1 and volumetric productivity of 9.5 mM h−1, suggesting a new potential approach for the industrial production of succinate.

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Keywords
Succinate; Oxygen deprivation; Two-stage process
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A two-stage process for succinate production using genetically engineered Corynebacterium acetoacidophilum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1692–1700
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us